Measurement of intracellular Ca2+ with confocal microscopy.
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چکیده
منابع مشابه
Confocal microscopy of intracellular calcium dynamics during fertilization.
During fertilization in all animals investigated, fully developed eggs or meiotically immature oocytes must generate a proper change in their intracellular free calcium concentrations ([Ca]i) to develop normally (31). The first images of a fertilization-induced calcium response were obtained in fish oocytes that had been microinjected with the calcium-sensitive photoprotein, aequorin (4). Since...
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The regulation of cytosolic Ca(2+) homeostasis is essential for cells, including vascular smooth muscle cells. Arterial tone, which underlies the maintenance of peripheral resistance in the circulation, is a major contributor to the control of blood pressure. Diuretics may regulate intracellular Ca(2+) concentration ([Ca(2+)](i)) and have an effect on vascular tone. In order to investigate the ...
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Confocal fluorescence microscopy and atomic force microscopy (AFM) are two established techniques that can be used to study single molecules. Both techniques have their individual capabilities that permit to study different aspects such as topography using AFMs, or dynamics using fluorescence spectroscopy. Thus, a combination of both techniques into a single instrument enhances the methodology ...
متن کاملStandardization of corneal haze measurement in confocal microscopy.
PURPOSE Corneal stromal haze cannot be compared in longitudinal studies or across laboratories without standardization. In this study, a method was devised of standardizing image brightness in confocal microscopy of the cornea. METHODS Thirty-six normal corneas of 18 untreated volunteers and 35 corneas of 18 patients 3 years after LASIK were examined by slit scanning confocal microscopy. The ...
متن کاملConfocal Microscopy
A confocal microscope creates sharp images of a specimen that would otherwise appear blurred when viewed with a conventional microscope. This is achieved by excluding most of the light from the specimen that is not from the microscope’s focal plane. The image has less haze and better contrast than that of a conventional microscope and represents a thin cross-section of the specimen. Thus, apart...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 1991
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.31.34